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淋巴结生发中心的研究有其特殊性 ,即细胞组成相当复杂 ,采用常规的全组织研究往往不能针对所需要的细胞。1993年Ｈａｎｓｍａｎｎ等[1] 建立了一种从组织切片上提取单个淋巴细胞并进行聚合酶链反应 (ＰＣＲ)的研究方法。该方法的最大优点在于能够针对所需细胞进行相应的分子生
The study of lymph node germinal center has its own particularity, that is, the cell composition is quite complicated, and the conventional whole-tissue research often can not target the desired cells. In 1993, Hansmann et al. [1] established a method of extracting individual lymphocytes from tissue sections and performing polymerase chain reaction (PCR). The biggest advantage of this method is that it allows for the corresponding molecular production of the desired cells