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本文应用碱性洗脱荧光分析法探讨了槐定对互隔交链孢霉的两种毒素——交链孢酚单甲醚(AME)和交链孢酚(AOH)引起2BS细胞DNA单链断裂的抑制作用。实验结果表明,槐定能抑制AME和AOH引起的2BS细胞DNA单链断裂,加入不同浓度的槐定作用后洗脱9小时DNA在滤膜上的存留分数分别为:AME组,由对照组的0.205=0.02回升至0.57±0.02、0.61±0.05、0.74±0.03;AOH组,由对照组的0.11±0.01回升至0.45±0.04、0.58±0.03、0.85±0.06,实验组与对照组比较统计学处理均有显著差别(P<0.01)。
In this paper, the alkaline elution fluorescence assay was used to investigate the effect of the two toxins of chlorimidine on Alternaria alternifolia, acibenzophris monomethyl ether (AME) and cyclosporine (AOH), to induce DNA single strands in 2BS cells. Inhibition of fracture. The experimental results showed that buprenorphine inhibited DNA single-strand breaks caused by AME and AOH in 2BS cells, and the fractions of DNA eluted on membrane after 9-hour elution with different concentrations of thiazepam were: AME group, from the control group 0.205 = 0.02 increased to 0.57 ± 0.02, 0.61 ± 0.05, and 0.74 ± 0.03; AOH group increased from 0.11 ± 0.01 in the control group to 0.45 ± 0.04, 0.58 ± 0.03, and 0.85 ± 0.06. The experimental group was statistically treated as compared with the control group. There were significant differences (P<0.01).