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为证实正常大鼠肾皮质有高、低亲和力糖皮质激素受体（ＧＲＨ、ＧＲＬ）。方法利用放射配体结合法。结果ＧＲＬ的平衡解离常数（ｋｄ）在１０－６Ｍ数量级，最大结合容量（Ｂｍａｘ）为８．６０±１．３０ｐｍｏｌ／ｍｇ．ｐｒｏ。被动性海曼氏肾炎（ＰＨＮ组）大鼠ＧＲＬ的Ｂｍａｘ为１０．７±３．４０ｐｍｏｌ／ｍｇ．ｐｒｏ。经小剂量（ＬＤ组）、大剂量（ＨＤ组）糖皮质激素（ＧＣ）治疗ＰＨＮ大鼠后，其ＧＲＬ的Ｂｍａｘ分别增加３倍左右，ＧＣ对ＧＲＬ无反向调节。ＨＤ组同ＬＤ组比，血ＧＣ浓度高，可和相当数量的ＧＲＬ结合而起到抗炎效应，从而疗效好。ＨＤ组大剂量ＧＣ治疗后２４ｈ尿蛋白减少量与肾皮质ＧＲＬ的Ｂｍａｘ呈正相关。结论大剂量ＧＣ可能通过ＧＲＬ介导而起到强有力的抗炎效应
In order to confirm the normal rat renal cortex with high and low affinity glucocorticoid receptor (GRH, GRL). Method using radioligand binding method. Results The equilibrium dissociation constant (kd) of GRL was in the order of 10-6M and the maximum binding capacity (Bmax) was 8.60 ± 1.30pmol / mg. pro. The Bmax of Passive Hahn Nephritis (PHN group) rats was 10.7 ± 3.40 pmol / mg. pro. After treatment of PHN rats with low dose (LD group) and high dose (HD group) glucocorticoid (GC), the Bmax of GRL increased by about 3-fold, respectively. There was no reverse regulation of GRL by GC. Compared with LD group HD group, blood GC concentration, and a considerable amount of GRL combined anti-inflammatory effect, which has good curative effect. The decrease of urinary protein in HD group was positively correlated with Bmax of renal cortex GRL after high-dose GC treatment. Conclusion High-dose GC may play a powerful anti-inflammatory effect through GRL