TRAIL蛋白的体外活性稳定性初步研究

来源 :工业微生物 | 被引量 : 0次 | 上传用户：rwteng

【摘要】

：

大肠杆菌发酵表达的TRAIL蛋白经过两步纯化可得到纯度95%以上的活性蛋白,得率为40%.天然TRAIL蛋白为同源三聚体形式,在三聚体中心隐蔽结合Zn可维持TRAIL蛋白天然结构的稳定性

【作者】

：

张红魏东芝周文瑜陶欣艺沈亚领周劲松张国钧孙菁

【出处】

：

工业微生物

【发表日期】

：

2004年期

【关键词】

：

TRAIL 纯化 Zn2+ L-Arg L-Glu 蛋白稳定性

【基金项目】

：

上海市重点学科建设项目

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大肠杆菌发酵表达的TRAIL蛋白经过两步纯化可得到纯度95%以上的活性蛋白,得率为40%.天然TRAIL蛋白为同源三聚体形式,在三聚体中心隐蔽结合Zn可维持TRAIL蛋白天然结构的稳定性.研究发现在纯化后的重组可溶性TRAIL蛋白中添加Zn与TRAIL单体的摩尔比例达到2:1时,TRAIL蛋白的活性达到最高.本文初步研究了TRAIL蛋白的体外活性稳定性问题,发现在添加了最适Zn

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TRAIL蛋白的体外活性稳定性初步研究

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