PAM染色法是观察肾小球基底膜的重要方法之一。我们选择不同的温度对肾穿刺组织切片进行水浴PAM染色,结果发现,提高水浴温度可缩短染色时间且效果较好。1材料与方法1.1材料南京军区南京总医院病理科肾穿刺标本30例。1.2试剂与设备①六胺银储备液:3%六次甲基四胺水溶液100ml;5% PAM staining is one of the most important ways to observe glomerular basement membrane. We choose a different temperature for renal puncture tissue sections were PAM bath water staining and found that increasing the water bath temperature can shorten the dyeing time and better. 1 Materials and Methods 1.1 Materials Nanjing General Hospital of Nanjing Military Region, 30 cases of renal biopsy specimens. 1.2 Reagents and equipment ① hexamine silver stock solution: 3% hexamethylenetetramine aqueous solution 100ml; 5%