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目的探讨高原低氧对大鼠慢性炎症的影响。方法 40只SD大鼠随机均分为正常对照组(对照组)、慢性炎症组(慢炎组)、高原低氧组(高低组)、高原低氧+慢性炎症组(高+慢组);慢炎组大鼠尾静脉注射脂多糖(LPS,0.5 mg/kg),每周2次,连续注射4周,高+慢组经慢炎组相同处理后,同高低组大鼠一起置于模拟海拔6000m的高原低氧环境中连续3 d;然后进行肺组织取材、病理切片HE染色观察。外周血白细胞分类和计数,酶联免疫吸附实验(ELISA)分别检测血清和肺组织IL-6、TNF-α水平,Western印迹检测肺组织IL-6蛋白表达。结果经LPS和高原低氧暴露处理过的大鼠肺组织出现炎细胞浸润、肺泡间毛细血管扩张的病理学变化;外周血白细胞分类和计数。ELISA结果显示,与对照组相比,慢炎组、高低组均能导致大鼠外周血白细胞总数增加(P<0.01),血清和肺组织IL-6和TNF-α水平均升高(均P<0.05);高原低氧和慢性炎症具有交互作用(P<0.01),高+慢组进一步增加了外周血白细胞数,并促进IL-6和TNF-α的表达升高(P<0.01)。结论通过大鼠脂多糖慢性炎症模型,证实高原低氧可加重慢性炎症发展。
Objective To investigate the effect of plateau hypoxia on chronic inflammation in rats. Methods Forty SD rats were randomly divided into four groups: control group, chronic inflammation group, hypoxia group and hypoxia + chronic inflammation group. The rats in the slow inflammation group were injected LPS (0.5 mg / kg) into the caudal vein twice a week for 4 weeks. The rats in the high + slow group were given the same treatment as the slow inflammation group, Altitude of 6000m plateau hypoxia environment for 3 days; then the lung tissue drawn, pathological sections HE staining observed. The levels of IL-6 and TNF-α in serum and lung tissue were detected by enzyme linked immunosorbent assay (ELISA). The expression of IL-6 protein in lung tissue was detected by Western blotting. Results Inflammatory cells infiltration, pathological changes of alveolar telangiectasia, classification and counting of peripheral leukocytes were observed in lungs of rats exposed to LPS and altitude hypoxia. The results of ELISA showed that compared with the control group, the levels of IL-6 and TNF-α in the chronic inflammation group and the high and low groups all increased (P <0.01) and the levels of IL-6 and TNF-α in the serum and lung tissue increased <0.05). The plateau hypoxia interacted with chronic inflammation (P <0.01). The number of peripheral blood leukocytes and the expression of IL-6 and TNF-α were increased in high + slow group (P <0.01). Conclusion Through the model of chronic lipopolysaccharide-induced chronic inflammation in rats, it is confirmed that hypoxia in the plateau aggravates the development of chronic inflammation.