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目的分析新疆细粒棘球蚴AgB1、AgB2、AgB4蛋白的氨基酸序列,了解其蛋白二级结构特点,预测抗原表位,为包虫病的进一步免疫学诊断和研究提供理论支持。方法利用生物信息分析软件DNAstar分析3种抗原的蛋白二级结构特点,运用在线软件IEDB对3种抗原各参数进行综合分析并预测其抗原表位。结果EgAgB1、EgAgB2和EgAgB4分别是由78、90、91个氨基酸残基组成的不同多肽。其蛋白质二级结构中均有α-螺旋、β-折叠、转角区域和卷曲区域。综合分析各参数推测EgAgB1具有2个抗原表位,分别是14~23位氨基酸残基(DDGLTSTSRS)、37~42位氨基酸残基(RDPLGQ)。推测EgAgB2的2个抗原表位分别为41~49位氨基酸残基(DFFRNDPLG)、83~90位氨基酸残基(EEKDDDSk)。EgAgB4可能含有的2个抗原表位分别是43~50位氨基酸残基(RSDPLGQR)、84~89位氨基酸残基(EEEDDS)。结论采用生物信息技术对新疆细粒棘球蚴AgB1、AgB2、AgB4进行B细胞表位特点的研究和分析,对更准确诊断包虫病具有重要意义。
Objective To analyze the amino acid sequence of AgB1, AgB2 and AgB4 of Echinococcus granulosus in Xinjiang and to understand the secondary structure of the protein and to predict the epitopes of Echinococcus granulosus, and to provide theoretical support for further immunological diagnosis and research of hydatid disease. Methods The bioinformatics analysis software DNAstar was used to analyze the secondary structure of the three antigens. The online software IEDB was used to analyze the three antigens and to predict their epitopes. Results EgAgB1, EgAgB2 and EgAgB4 were different polypeptides consisting of 78,90,91 amino acid residues, respectively. Its secondary structure of proteins are α-helix, β-sheet, corner area and curling area. According to the comprehensive analysis of each parameter, we speculated that EgAgB1 has two epitopes: DDGLTSTSRS (amino acid residues 14-23) and amino acid residues 37-42 (RDPLGQ). The two epitopes of EgAgB2 were deduced to be 41-49 amino acid residues (DFFRNDPLG) and 83-90 amino acid residues (EEKDDDSk), respectively. The two epitopes that EgAgB4 may contain are amino acid residues 43-50 (RSDPLGQR) and amino acid residues 84-89 (EEEDDS), respectively. Conclusion The bioinformatics analysis and characterization of B cell epitopes of Echinococcus granulosus AgB1, AgB2 and AgB4 in Xinjiang is of great significance for more accurate diagnosis of hydatid disease.