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目的了解登封市屠宰场生猪小肠结肠炎耶尔森菌带菌率、血清分型、毒力基因分布等情况,为制定预防措施提供参考依据。方法从屠宰场生猪扁桃体及回盲部内容物中分离小肠结肠炎耶尔森菌,并进行生化鉴定、血清分型、毒力基因PCR检测。结果从196份生猪咽拭子中分离出107株小肠结肠炎耶尔森菌,检出率为54.59%;从196份生猪肛拭子中分离出36株小肠结肠炎耶尔森菌,检出率为18.37%。检出菌血清型为O∶3,生物型为3型,Ⅰ型(ail+、ystA+、ystB-、yadA+、virF+)占93.71%(134/143),Ⅱ型(ail+、ystA+、ystB-、yadA-、virF-)占6.29%(9/143)。结论登封市生猪小肠结肠炎耶尔森菌以致病性菌株为主,今后应加强该菌的进一步监测工作。
Objective To understand the incidence of Yersinia enterocolitica infection, serotyping and virulence gene distribution in live pigs in the slaughterhouse in Dengfeng City, and to provide a reference for the formulation of preventive measures. Methods Yersinia enterocolitica was isolated from the contents of tonsil and ileocecal part of slaughterhouse and biochemical identification, serotyping and virulence gene PCR. Results 107 strains of Yersinia enterocolitica were isolated from 196 swine throat swabs and the detection rate was 54.59%. Thirty-six strains of Yersinia enterocolitica were isolated from 196 swine anal swabs and detected The rate was 18.37%. The serotypes were O: 3 and biotype 3, with 93.71% (134/143) of type Ⅰ (ail +, ystA +, ystB-, yadA -, virF-) accounted for 6.29% (9/143). Conclusion Dengfeng City, Yersinia enterocolitica pathogens mainly, further monitoring of the bacteria should be strengthened in the future.