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采用一种新的真核细胞表达载体ｐＥＦ－ＢＯＳ在ＣＯＳ细胞内表达了ｇｐ１３０和ＧＡＭ两种分子，并利用免疫沉淀和Ｗｅｓｔｅｒｎ－ｂｌｏｔ观察了表达结果。发现应用ｐＥＦ－ＢＯＳ表达载体可在ＣＯＳ细胞内获得上述两种分子的较高表达，优于一般常用的ｐＳＶ表达载体。
A new eukaryotic cell expression vector pEF-BOS was used to express gp130 and GAM in COS cells. The expression of Gp130 and GAM was observed by immunoprecipitation and Western-blot. It was found that the pEF-BOS expression vector obtained higher expression of these two molecules in COS cells, which is superior to the commonly used pSV expression vector.