论文部分内容阅读
目的 :观察卡介苗接种对高盐负荷及肾动脉狭窄所致高血压的影响 ,为寻找防治人类高血压的新方法提供实验依据。方法 :以高盐食物喂饲及肾动脉狭窄术分别制作高盐性高血压 (SH)及肾性高血压 (RH)模型。高盐喂饲或肾动脉狭窄术后 4周 ,选择高血压大鼠及正常对照大鼠进行实验。所有大鼠腹部皮下接种卡介苗一次 ,接种前及接种后每周测定血压变化情况 ,同时每时段收集 2 4h尿样并以Greiss反应法进行一氧化氮终产物NO-2 及NO-3 (UNOx)的测定。 3周后大鼠分别取主动脉组织并用Westernblot法检测iNOS蛋白水平。结果 :卡介苗接种对正常大鼠血压无明显影响。但接种 1周时 ,能使高血压大鼠的血压明显下降。其中尤以卡介苗接种 +L -精氨酸作用明显。NO测定表明 ,卡介苗接种后体内NO生成明显增加。Westernblot结果证明卡介苗接种后 ,高血压大鼠主动脉血管组织中iNOS蛋白明显高于正常大鼠。结论 :卡介苗接种能通过激活iNOS/NO途径降低某些类型高血压大鼠的血压
OBJECTIVE: To observe the effect of BCG vaccination on hypertension caused by high salt load and renal artery stenosis, and to provide experimental evidence for finding new ways to prevent and treat human hypertension. Methods: High-salt hypertensive (SH) and renal hypertensive (RH) models were made by high-salt diet and renal artery stenosis. Four weeks after high-salt feeding or renal artery stenosis, hypertensive rats and normal control rats were selected for experiment. All rats were inoculated intraperitoneally once a day with BCG, and blood pressure was measured weekly before and after inoculation. At the same time, urine samples were collected for 24 hours and nitric oxide endproducts NO-2 and NO-3 (NOOx) The determination. After 3 weeks, the aorta tissues were taken and the iNOS protein levels were detected by Western blot. Results: BCG vaccination had no significant effect on blood pressure in normal rats. However, one week after inoculation, the blood pressure of hypertensive rats can be obviously decreased. Especially in BCG vaccination + L - arginine obvious effect. NO measurement showed that after BCG vaccination in vivo NO production increased significantly. Western blot results showed that after BCG vaccination, iNOS protein in aortic vascular tissue of hypertensive rats was significantly higher than that of normal rats. CONCLUSION: BCG vaccination can reduce the blood pressure in some types of hypertensive rats by activating the iNOS / NO pathway