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混合物光谱中组分数的确定是分析化学中常要遇到的问题。因子分析方法是对一组混合物样品的数据矩阵进行特征值分析,从而确定组分数。这种方法在实际应用时有很大的局限性,例如对一个待测的多组分未知样品是无法配制一系列含有同样组分而又有不同的相对浓度,个数掇于组分数的样品。三维荧光光谱是随激发,发射波长同时变化的荧光光谱。对应于每一对激发,发射波长的各个相对荧光强度,构成了荧光的激发—发射矩阵(EEM)。它的不同行对应于不同的荧光激发波长,不同列对应不同的发射波长。一个荧光试样就可以有一个EEM。
The determination of the number of components in the spectrum of a mixture is a problem often encountered in analytical chemistry. Factorial analysis is the eigenvalue analysis of the data matrix of a mixture of samples to determine the number of components. This method has great limitations in practical application. For example, it is impossible to formulate a series of samples containing the same components but different relative concentrations, the number of which is in the group of components, for an unknown sample of multiple components to be tested . The three-dimensional fluorescence spectrum is the fluorescence spectrum that varies with excitation and emission wavelength. Each relative fluorescence intensity corresponding to each pair of excitation and emission wavelengths constitutes the fluorescence excitation-emission matrix (EEM). Its different rows correspond to different fluorescence excitation wavelengths, different columns correspond to different emission wavelengths. A fluorescent sample can have an EEM.