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目的：利用稳定表达ＣＤ８ε融合分子的细胞凋亡模型，研究其ＣＤ３εＩＴＡＭ中两个酪氨酸的突变对细胞凋亡信号传递及相关基因表达的影响。方法：将稳定表达ＣＤ８ε融合分子及其３种突变分子的Ｔ淋巴细胞分别用抗ＣＤ８单抗刺激后，检测４种细胞蛋白酪氨酸磷酸化和胞浆Ｃａ２＋浓度的变化以及细胞凋亡相关基因表达。结果：抗体刺激后，表达ＣＤ８ε的细胞与表达其３种突变分子的细胞相比，其蛋白磷酸化增加，胞浆Ｃａ２＋浓度升高；立早基因ｎｕｒ７７和细胞凋亡基因ｆａｓ表达水平升高。结论：首次发现ＣＤ３εＩＴＡＭ中两个酪氨酸的突变阻断了ＣＤ３介导的凋亡信号传导途径，并影响ｎｕｒ７７和ｆａｓ基因的表达。
OBJECTIVE: To study the effect of two tyrosine mutations in CD3εITAM on apoptosis signal transduction and related gene expression by using the cell apoptosis model which stably expresses CD8ε fusion molecule. Methods: T lymphocytes stably expressing CD8ε fusion molecule and its three mutant molecules were respectively stimulated with anti-CD8 monoclonal antibody to detect the changes of protein tyrosine phosphorylation and cytoplasmic Ca2 + concentration in four cell types and the expression of apoptosis-related genes expression. Results: Compared with the cells expressing CD8 epsilon, the phosphorylation of CD8 epsilon increased and the cytosolic Ca2 + concentration increased. The expression level of nur77 and apoptotic gene fas increased. CONCLUSION: It was first found that the mutation of two tyrosines in CD3εITAM blocked the CD3-mediated apoptotic signal transduction pathway and the expression of nur77 and fas genes.