论文部分内容阅读
目的:初步比较人牙周膜干细胞(human periodontal ligament stem cell,PDLSC)在牙根不同发育阶段时的增殖能力和成牙/成骨能力的差异,为组织工程化牙齿的种子细胞来源提供一定的实验基础。方法:分别分离培养来自人根尖孔未闭合及根尖孔完全闭合牙齿的PDLSC,通过MTT法检测其增殖能力,碱性磷酸酶(alkaline phosphatase,ALP)试剂盒法检测其ALP活性,茜素红染色法检测两者的体外矿化能力,Western blot检测其牙本质基质蛋白1(dentin matrix protein 1,DMP1)、牙本质涎蛋白(dentin sialoprotein,DSP)、核心结合因子(runt-related transcription factor 2,RUNX2)和骨细胞特异性转录因子(osterix,OSX)的蛋白表达情况。结果:来自人根尖孔未闭合牙的PDLSC的增殖活性、ALP活性和矿化能力、DMP1、RUNX2、DSP和OSX蛋白的表达量均高于根尖孔完全闭合牙的PDLSC,差异具有统计学意义(P<0.05)。结论:PDLSC的增殖和成牙/成骨能力随着牙根发育阶段的不同而变化,随着牙根发育的完全,PDLSC的增殖能力和成牙/成骨能力均下降,这种影响可能是由于牙根不同发育阶段牙根周围微环境的变化而导致的。
OBJECTIVE: To compare the proliferation and odontogenic / osteogenic abilities of human periodontal ligament stem cells (PDLSCs) at different developmental stages of tooth root, and to provide some experiments for the source of seed cells of tissue-engineered teeth basis. Methods: The PDLSCs from the apical foramen ovale and the apical foramen were respectively isolated and cultured. The proliferation of PDLSCs was detected by MTT assay. ALP activity was detected by alkaline phosphatase (ALP) The mineralization ability of both in vitro and in vivo was examined by red staining. The expression of dentin matrix protein 1 (DMP1), dentin sialoprotein (DSP), runt-related transcription factor 2, RUNX2) and osteoblast-specific transcription factor (osterix, OSX) protein expression. Results: The proliferative activity, ALP activity and mineralization ability of PDLSCs from human apical foramen were higher than those from PDLSC with complete closure of apical foramen. The difference was statistically significant Significance (P <0.05). CONCLUSION: PDLSC proliferation and odontoblast / osteogenesis vary with different stages of root development. With the complete root development, PDLSC proliferation and odontoblading / osteogenic ability both decreased, which may be due to root Different developmental stages around the root microenvironment caused by changes.