论文部分内容阅读
本文研究脂质体和微球包裹的霍乱弧菌抗原及其接种途径对诱导免疫应答的作用。 抗原性蛋白取自EITor生物型霍乱弧菌KB207株,通过高效液相色谱分级分离制得无细胞溶解产物,分别用可生物降解的脂质体和聚乳酸聚羟基乙酸微球（PLGA）包裹此抗原。脂质体以卵磷酯和胆固醇混合后通过超声处理制备。用游离抗原和包裹抗原经
In this paper, the liposome and microsphere-encapsulated Vibrio cholerae antigens and their routes of vaccination on the induction of immune response. The antigenic protein was obtained from the strain of VITC Vibrio cholerae KB207, and the cell-free lysate was fractionated by high performance liquid chromatography (HPLC). The cells were coated with biodegradable liposomes and polylactic acid polyglycolic acid microspheres (PLGA) antigen. Liposomes were prepared by sonication after mixing lecithin and cholesterol. With free antigen and antigen package by