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通过前瞻性研究对100例经临床诊断和手术证实为耳硬化症的患者与100例年龄、性别相同的无耳硬化症的人进行了HLA分型对比,以研究耳硬化症与HLA类型的关系。方法:按Mackintosh介绍的方法做标准的小淋巴细胞毒性分析,收集10ml肝素抗凝血,分离单核细胞,通过缓慢离心去除血小板,经
A prospective study of 100 cases of patients diagnosed as otosclerosis by clinical diagnosis and surgery and 100 age-related and gender-free patients with otosclerosis HLA typing comparison to study the relationship between otosclerosis and HLA type . Methods: According to the method described by Mackintosh standard small lymphocytotoxicity analysis, collecting 10ml heparin anticoagulant, monocytes isolated by slow centrifugation to remove platelets by