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目的评价磷脂酰肌醇蛋白聚糖3(GPC3)、血管内皮生长因子(VEGF)、转移抑制因子(nm23)在肝细胞癌转移中的诊断价值。方法采用免疫组织化学技术检测3标记物在54例HCC组织(27例HCC转移、27例HCC未转移)中的表达,比较单项和多项蛋白联合检测的准确性。结果 3标记物在HCC转移组和未转移组中阳性表达率差异有统计学意义(P<0.05)。单项检测GPC3、VEGF、nm23的ROC曲线下面积(AUC)为0.646(P<0.05)、0.838(P<0.05)、0.678(P<0.05)。任意2指标联合检测有助于提高HCC转移组的检出率,其中以GPC3和nm23组合最能提高诊断敏感性和特异性,3指标联合应用时,检出率达100%。结论合理选择多指标联合检测对HCC复发转移的判断有重要意义。
Objective To evaluate the diagnostic value of glypican 3 (GPC3), vascular endothelial growth factor (VEGF) and metastasis suppressor (nm23) in the metastasis of hepatocellular carcinoma. Methods Immunohistochemical technique was used to detect the expression of 3 markers in 54 HCC tissues (27 HCCs and 27 HCCs without metastasis). The accuracy of the combined detection of single and multiple proteins was compared. Results The positive rates of 3 markers in HCC metastasis group and non-metastasis group were significantly different (P <0.05). The area under the ROC curve of single detection of GPC3, VEGF and nm23 were 0.646 (P <0.05), 0.838 (P <0.05) and 0.678 (P <0.05). The combination of any two indexes could improve the detection rate of HCC metastasis group. The combination of GPC3 and nm23 could improve the sensitivity and specificity of diagnosis. The detection rate was 100% when the three indexes were combined. Conclusion A reasonable choice of multi-index joint detection of HCC recurrence and metastasis of great significance.