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曾在一个儿童患者体内，发现一个新的乙型肝炎病毒（ＨＢＶ）变异株，其ＨＢｓＡｇ主蛋白ａａ１２６发生Ｉｌｅ（ＡＴＴ）到Ｓｅｒ（ＡＧＴ）的取代。已知ａｄｒ／ａｙｒ亚型ＨＢｓＡｇ１２６位为Ｉｌｅ，而ａｄｗ／ａｙｗ亚型ＨＢｓＡｇ１２６位为Ｔｈｒ，表明ＨＢｓＡｇ１２６Ｓｅｒ是一个新的变异株。用计算机做结构分析的结果表明，突变体ＨＢｓＡｇ１２６Ｓｅｒ主蛋白ａａ１２０－ａａ１３０区段的二级结构与野生型ａｄｒＨＢＶＨＢｓＡｇ１２６Ｉｌｅ相比发生明显的改变。这种构象的变化可能会影响ａ抗原决定簇（ａ１２４－ａａ１４７）的抗原性。为了证实这一点，构建了突变Ｓ基因表达质粒，在ＳＶ４０早期启动子的控制下进行抗原表达。利用ＨＢｓＡｇ９肽（Ｔｈｒ－Ｉｌｅ１２６－Ｐｒｏ－Ａｌａ－Ｇｌｎ－Ｇｌｙ－Ｔｈｒ－Ｓｅｒ－Ｍｅｔ）抗ｉ单克隆抗体和９肽（Ｔｈｒ－Ｔｈｒ１２６－Ｐｒｏ－Ａｌａ－Ｇｌｎ－Ｇｌｙ－Ｔｈｒ－Ｓｅｒ－Ｍｅｔ）抗ｔ单克隆抗体进行放射免疫测定，结果表明，这种Ｓｅｒ１２６突变蛋白对抗ｉ单克隆抗体的反应性比野生蛋白减弱，对抗ｔ单克隆抗体的反应性则与ＨＢｓＡｇ１２６Ｔｈｒ接近。但用三种抗－ａ单克隆抗体的检测结果揭示，突变蛋白ＨＢｓＡｇ１２６?
In a pediatric patient, a new variant of the hepatitis B virus (HBV) was found in which the HBsAg aa126 was substituted with Ile (ATT) to Ser (AGT). The adr / ayr subtype of HBsAg126 is known to be Ile, while the adw / ayw subtype of HBsAg126 is Thr, indicating that HBsAg126Ser is a new variant. Computer structural analysis showed that the secondary structure of the mutant aa120-aa130 HBsAg126Ser main protein compared with the wild-type adrHBVHBsAg126Ile significantly changed. This conformational change may affect the antigenicity of the a-antigenic determinant (a124-aa147). To confirm this, a mutant S gene expression plasmid was constructed and antigenic expression was performed under the control of the SV40 early promoter. Anti-i monoclonal antibody and 9 peptide (Thr-Thr126-Pro-Ala-Gln-Gly-Thr-Ser-Met) t monoclonal antibody radioimmunoassay, the results showed that the Ser126 mutein anti-i monoclonal antibody reactivity than the reduced wild-type protein, anti-t monoclonal antibody reactivity and HB sAg126Thr close. However, the results of the three anti-a monoclonal antibodies revealed that the mutant protein HBsAg126?