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粘虫颗粒体病毒经０．０２ｍｏｌ／ＬＮａＯＨ碱溶，先用ＳｅｐｈａｄｅｘＧ－２００凝胶过滤层析柱从病毒蛋白粗提物中分离增效因子，然后选用ＤＥＡＥ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ离子交换层析柱进一步纯化增效因子，得到少量电泳纯的增效因子蛋白样品。纯化的增效因子具有典型的蛋白紫外吸收光谱，最大吸收波长为２７５．２ｎｍ。ＳＤＳ－ＰＡＧＥ电泳确定增效因子的分子量为１０８ｋＤ。用苏丹黑Ｂ脂蛋白染色法、过碘酸－Ｓｃｈｉｆ试剂糖蛋白染色法定性分析增效因子，非变性电泳凝胶上的增效因子带均为阴性反应。等电聚焦电泳检测增效因子的等电点为ｐＨ５．３左右。氨基酸组成分析证明，增效因子富含酸性氨基酸，占蛋白总量的２６．４２％，碱性氨基酸的含量较低
The armyworm particle virus was dissolved in 0.02mol / L NaOH. SephadexG-200 gel filtration column was used to separate the synergistic factor from the crude virus protein extract. Then, DEAE-Sepharose CL-6B ion exchange chromatography Purification efficiency factor, a small amount of electrophoresis pure efficiency factor protein samples. The purified efficiency factor has a typical protein UV absorption spectrum, the maximum absorption wavelength of 275.2nm. The molecular weight of the synergistic factor was 108kD by SDS-PAGE electrophoresis. Sudan B lipoprotein staining method, periodic acid - Schif reagent glycoprotein staining method for the qualitative analysis of synergistic factor, non-denaturing electrophoresis gel on the efficiency factor band were negative. Isoelectric focusing electrophoresis to detect the synergistic factor has an isoelectric point of about pH5.3. Amino acid composition analysis proved that the synergistic factor is rich in acidic amino acids, accounting for 26.42% of the total protein, the content of basic amino acids is low