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目的 :探明家禽家畜的O15 7∶H7感染与饲养情况。方法 :用细菌学与PCR等技术检测O15 7∶H7及其毒力基因。结果 :从牛、羊、猪、鸡的粪便中检出O15 7∶H7及其毒力基因VT2 、eaeA和hly ,带菌率的高低依次为牛 >羊 >鸡 >猪 ,腹泻病流行前期禽畜的带菌率高于流行期 ;该地农村禽畜饲养密度高 ,户饲养数 10 .2只 (头 ) ,人均 2 .4只 (头 ) ,饲养方式以散养为主 ,散养率 87.76%。结论 :牛、羊、猪、鸡是该地O15 7∶H 7的主要宿主动物和传染源 ,加强对其实行良好的饲养方式与粪便无害化管理及注重个人与环境卫生是防治O15 7∶H7引起感染性腹泻的关键
Objective: To identify the infection and rearing status of O15 7:H7 in poultry and livestock. Methods: Bacteriological and PCR techniques were used to detect O15 7:H7 and its virulence genes. Results: O15 7:H7 and its virulence genes VT2, eaeA and hly were detected in the feces of cattle, sheep, pigs and chickens, and the rates of infection were as follows: cattle> sheep> chicken> pigs, pre-epidemic period of diarrhea The rate of carrying bacteria was higher than that of epidemic period. The livestock breeding density in rural areas was high with 10.2 households (head) and 2. 4 persons (head) . Conclusion: Cattle, sheep, pigs and chickens are the major host animals and sources of infection in O15 7:H 7 in this area. They should be strengthened to implement good feeding practices and harmless management of faeces and pay attention to personal and environmental hygiene. O15 7: H7 causes infectious diarrhea