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本研究的目的是从骨髓增生异常综合征-慢性粒-单核细胞白血病(MDS-CMML)病人获取骨髓细胞,建立 非生长因子依赖的细胞株。该细胞株在不添加任何生长因子的含15％胎牛血清的RPMI 1640和DMEM混合培养 液中进行了培养,并分别从细胞株的形态、细胞表面抗原分子、细胞增殖、分化、凋亡等方面检测其生物学特性。结 果表明:在不添加任何生长因子的培养条件下该细胞株可以长期存活和生长,并能向单核及巨核细胞分化。结论: 成功建立了一株非生长因子依赖的MDS-JSN04(MDS-江苏南京04)细胞株,并阐明了其部分生物学特性。
The aim of this study was to obtain bone marrow cells from patients with myelodysplastic syndrome-chronic myelomonocytic leukemia (MDS-CMML) and establish a non-growth factor dependent cell line. The cell lines were cultured in RPMI 1640 and DMEM mixed medium containing 15% fetal bovine serum without any addition of growth factors and were cultured from cell lines in terms of morphology, cell surface antigen molecules, cell proliferation, differentiation, apoptosis, and the like Aspects to test its biological characteristics. The results showed that the cell line could survive and grow for a long time and could differentiate into monocytes and megakaryocytes under culture conditions without any growth factors. Conclusion: A non-growth factor dependent MDS-JSN04 (MDS-Jiangsu Nanjing 04) cell line was successfully established and its biological characteristics were elucidated.