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以剑麻H.11648为材料,研究外植体类型、激素种类及其浓度对剑麻愈伤组织的诱导和植株再生的影响,并且建立剑麻茎尖愈伤组织诱导及高频再生体系。结果表明:MS基本培养基、6-BA/NAA激素组合有利于淡黄绿色愈伤组织的形成,SH基本培养基、6-BA/IBA/NAA激素组合有利于不定芽的分化;最佳愈伤组织诱导和分化培养基分别为MS+3.0 mg/L 6-BA+0.2 mg/L NAA,SH+5.0 mg/L 6-BA+0.1 mg/L NAA+0.1 mg/L IBA。在该培养条件下,H.11648麻茎尖愈伤组织的诱导率为86.67%,分化率为(98.33±1.67)%,分化系数为(13.19±0.58)。再生植株在不加激素的MS基本培养基上培养4周后的生根率为100%,平均生根数为(5.39±0.70)条,根长为(8.44±0.25)cm,该研究结果为剑麻转基因的研究和种质创新奠定了基础。
Sisal hemp H.11648 was used to study the effects of explant types, hormone types and concentrations on callus induction and plant regeneration of sisal hemp, and the induction and high frequency regeneration system of sisal callus stem callus was established. The results showed that the combination of MS basal medium and 6-BA / NAA hormone was beneficial to the formation of yellowish-green callus. SH basic medium and 6-BA / IBA / NAA hormone combination were conducive to the differentiation of adventitious buds. The tissue culture medium was MS + 3.0 mg / L 6-BA + 0.2 mg / L NAA, SH + 5.0 mg / L 6-BA + 0.1 mg / L NAA + 0.1 mg / L IBA. Under this culture condition, the induction rate of calli of H.11648 callus was 86.67%, the differentiation rate was (98.33 ± 1.67)% and the differentiation coefficient was (13.19 ± 0.58). The rooting rate of the regenerated plants after 100% culture for 4 weeks on MS basal medium without hormone was 5.39 ± 0.70 and the root length was (8.44 ± 0.25) cm. The results of this study were sisal Transgenic research and germplasm innovation laid the foundation.