论文部分内容阅读
[目的]研究ZNF488 siRNA对电离辐射诱发的鼻咽癌CNE1侵袭迁移能力的影响。[方法]采用qRT-PCR和Western blot检测ZNF488 siRNA转染效率。采用划痕实验和Transwell侵袭实验检测电离辐射对鼻咽癌CNE1细胞侵袭迁移能力的影响。采用划痕实验和Transwell侵袭实验检测沉默ZNF488后,电离辐射诱发的CNE1细胞侵袭迁移能力是否改变。[结果 ]在mRNA和蛋白水平,实验组(siZNF488组)表达量均明显低于对照组(siRNA-Ctrl组),其中实验组ZNF488 mRNA水平为对照组的(0.54±0.12)倍(P=0.023)。划痕实验证明电离辐射明显增强鼻咽癌CNE1细胞迁移能力;Transwell侵袭实验检测到0Gy组侵袭细胞数为302.67±18.77,4Gy组侵袭细胞数为371.67±15.63,4Gy组侵袭细胞数为0Gy组(1.23±0.03)倍(P=0.006)。沉默ZNF488后,电离辐射诱发的CNE1细胞侵袭迁移能力明显减弱,这一功能的实现与上皮间质转化(EMT)进程的逆转息息相关。[结论]ZNF488 siRNA通过逆转EMT进程抑制鼻咽癌细胞CNE1电离辐射诱发的侵袭迁移能力。
[Objective] To investigate the effect of ZNF488 siRNA on invasion and migration of nasopharyngeal carcinoma CNE1 induced by ionizing radiation. [Method] The transfection efficiency of ZNF488 siRNA was detected by qRT-PCR and Western blot. Scratch assay and Transwell invasion assay were used to detect the effect of ionizing radiation on invasion and migration of nasopharyngeal carcinoma CNE1 cells. Scratch assay and Transwell invasion assay were used to detect whether the invasion and migration of CNE1 cells induced by ionizing radiation were changed after silencing ZNF488. [Results] The expression of ZNF488 mRNA in the experimental group (siZNF488 group) was significantly lower than that of the control group (siRNA-Ctrl group) at the mRNA and protein levels, and the level of ZNF488 mRNA in the experimental group was (0.54 ± 0.12) ). Scratch experiments showed that ionizing radiation significantly enhanced the migration ability of CNE1 cells. Transwell invasion assay showed that the number of invasive cells in 0Gy group was 302.67 ± 18.77, the number of invasive cells in 4Gy group was 371.67 ± 15.63, the number of invasion cells in G1G group was 0Gy group 1.23 ± 0.03) times (P = 0.006). After silencing ZNF488, the invasion and migration ability of CNE1 cells induced by ionizing radiation was significantly weakened, and the realization of this function was closely related to the reversal of the process of epithelial mesenchymal transition (EMT). [Conclusion] ZNF488 siRNA can inhibit the invasion and migration of nasopharyngeal carcinoma cell line CNE1 by reversing the EMT process.