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目的观察不同温和灸对瘙痒模型小鼠行为学及亚细胞形态的影响。方法将40只C57BL/6J小鼠随机分为空白组、模型组、治疗I组(38℃艾灸)和治疗Ⅱ组(46℃艾灸)4组,每组10只。采用背部皮下注射辣椒素法造模。造模成功后,选取治疗I、Ⅱ组小鼠“长强穴”“神阙穴”艾灸治疗一周。采用面颊部注射组胺法观察小鼠1 min内面颊部搔抓次数,并比较4组小鼠搔抓次数差异,分析温和灸治疗瘙痒症效果。透射电子显微镜(TEM)观察小鼠面颊和肛周皮肤细胞中液泡数量,分析温和灸对瘙痒模型小鼠亚细胞形态的影响。结果面颊部注射组胺后,4组小鼠1 min内面颊部搔抓次数比较,差异有统计学意义(P<0.01),两两比较结果显示,治疗I组搔抓(9.4±1.7)次和治疗Ⅱ组(8.3±1.2)次均少于模型组的(11.6±2.3)次(P<0.01)。电镜结果显示4组小鼠面颊皮肤中液泡数量差异无统计学意义(P>0.05),但肛周皮肤中液泡数量差异有统计学意义(P<0.05)。与模型组小鼠肛周皮肤实质细胞中液泡数量(30.00±9.17)比较,治疗I组(17.33±5.51)和治疗Ⅱ组(5.67±2.08)均减少(P<0.05);与治疗I组比较,治疗Ⅱ组小鼠肛周皮肤细胞中液泡数量减少(P<0.05)。两两比较结果显示,治疗I、Ⅱ组肛周皮肤细胞中液泡数量少于面颊部皮肤细胞(P<0.05)。结论温和灸对瘙痒模型小鼠的瘙痒症状具有明显的改善作用,可能与长期温和灸刺激减少皮肤细胞中的液泡数量有关。
Objective To observe the effects of different mild moxibustion on the behavior and subcellular morphology of pruritus model mice. Methods Forty C57BL / 6J mice were randomly divided into blank group, model group, treatment group I (38 ℃ moxibustion) and treatment group Ⅱ (46 ℃ moxibustion). Subcutaneous injection of capsaicin subcutaneous modeling. After the success of modeling, select the treatment of I, Ⅱ mice “long strong point ” “Shenque point ” moxibustion treatment for a week. Histamine injection into the cheeks was used to observe the number of scratches on the surface of the buccal area within 1 min, and the difference in scratching times among the 4 groups was compared. The effect of mild moxibustion on pruritus was analyzed. The number of vacuoles in mouse cheek and perianal skin cells was observed by transmission electron microscopy (TEM), and the effect of mild moxibustion on the subcellular morphology of pruritus model mice was analyzed. Results There was significant difference in facial buccal scratching scores among the four groups (P <0.01) after histamine injection into the buccal area. The results of pairwise comparisons showed that scratching in group I was (9.4 ± 1.7) times And treatment Ⅱ (8.3 ± 1.2) times less than the model group (11.6 ± 2.3) times (P <0.01). Electron microscopy results showed that there was no significant difference in the number of vacuoles in the cheek skin between the four groups (P> 0.05), but the number of vacuoles in perianal skin was statistically significant (P <0.05). Compared with the number of vacuoles in the perianal skin parenchyma cells in the model group (30.00 ± 9.17), the number of vacuoles in the perianal skin parenchyma cells of the model group was significantly decreased (17.33 ± 5.51) and that of the treatment group (5.67 ± 2.08) (P <0.05) , The number of vacuoles in perianal skin cells in treatment group Ⅱ decreased (P <0.05). The results of each comparison showed that the number of vacuoles in perianal skin cells in treatment group I and group II was less than that in cheek skin cells (P <0.05). Conclusions Mild moxibustion can improve the pruritus symptoms of pruritus model mice, which may be related to the reduction of the number of vacuoles in skin cells by long-term mild moxibustion.