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本研究建立可溶性人类白细胞抗原 I类 (sHLA I)检测方法 ,并探讨贮存血中sHLA I浓度变化的意义。采用ELISA双抗夹心法定量检测 6 0例正常广东人血清中sHLA I水平和 2 0例献血员成分血中sHLA I含量。结果表明 :采用本技术时 ,可溶性HLA I最低检测限为 2 .84ng ml,批内变异系数为 5 .80 % ,批间变异系数为 9.0 0 % ,回收率≥ 98 5 7% ,广东人sHLA I平均值为 (6 99.5 4± 36 0 .10 )ng ml。贮存 2 8天的RBC和随机供者血小板的sHLA I的浓度明显高于其它成分血 ,并且与成分血中残存的白细胞数和贮存时间有关。结论 :用ELISA法检测可溶性HLA I灵敏、特异、稳定 ,在临床输血过程中可考虑选择性输注含有不同浓度可溶性HLA I的成分血。
This study established a soluble human leukocyte antigen class I (sHLA I) detection methods, and to explore the significance of changes in serum sHLA I concentration. The level of sHLA I in serum of 60 normal Cantonese people and the level of sHLA I in blood of 20 donors were detected by ELISA double-antibody sandwich method. The results showed that the minimum detectable limit of soluble HLA I was 2.84 ng ml, the intra-assay CV was 5.80%, the inter-assay CV was 9.0 0%, the recovery was 98 5 7% I average (6 99.5 4 ± 36 0 .10) ng ml. Concentrations of sHLA I in RBCs stored on day 28 and platelets in randomized donors were significantly higher than those in other components and correlated with the number of remaining leukocytes in the blood and storage time. CONCLUSION: Soluble HLA-I is sensitive, specific and stable by ELISA. Selective infusion of soluble HLA-I containing components in clinical transfusion may be considered.