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头孢三嗪能迅速抑制[~3H]-DAP掺入大肠杆菌K_(12) DP_(50) Sup F,阻碍DAP参与该菌的胞壁形成与细菌生长.头孢三嗪与妥布拉霉素有抗菌协同作用.但头孢三嗪对[~3H]-亮氨酸掺入绿脓杆菌10118无明显抑制作用.妥布拉霉素对[~3H]-亮氨酸掺入绿脓杆菌呈明显抑制作用,抑制曲线随药物浓度不同而呈三相变化.头孢三嗪与妥布拉霉素合用[~3H]-亮氨酸掺入绿脓杆菌10118的作用比单用妥布拉霉素弱,但曲线亦呈三相变化.结果表明;头孢三嗪作用机理在于抑制胞壁前体物质粘肽的合成,而对蛋白质合成无明显影响;而头孢三嗪与妥布拉霉素协同作用机理可能是诱导或促进了细菌蛋白质的合成,而并非是增强了对细菌蛋白质合成的抑制作用.
Ceftriaxone rapidly inhibited the incorporation of [~ 3H] -DAP into Escherichia coli K_ (12) DP_ (50) Sup F and hinder DAP from participating in the cell wall formation and bacterial growth of the bacterium. Cefotriazine and tobramycin However, ceftriaxone showed no significant inhibitory effect on the incorporation of [~ 3H] - leucine into Pseudomonas aeruginosa 10118. Tobramycin significantly inhibited the incorporation of [~ 3H] - leucine into Pseudomonas aeruginosa The inhibitory curves showed three-phase changes with the concentration of the drug.Cetrytrazine combined with tobramycin [~ 3H] -leucine incorporation into Pseudomonas aeruginosa 10118 was weaker than that of tobramycin alone, But the curve also showed a three-phase change.The results showed that the mechanism of action of ceftriaxone is to inhibit the synthesis of muco-peptide, which is a precursor of the cell wall, and has no obvious effect on the protein synthesis. The synergistic action mechanism of ceftriaxone and tobramycin may be Is to induce or promote bacterial protein synthesis, but not to enhance the inhibition of bacterial protein synthesis.