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目的 :探讨鼻粘膜上皮细胞应答局部组织缺氧 ,合成分泌诱发型一氧化氮合酶 (iNOS)的变化 ,以及地塞米松对此的影响及意义。方法 :将人鼻粘膜上皮细胞在常氧和缺氧状态下进行无血清原代细胞培养 ,并加入不同浓度的地塞米松共同孵育 ,采用流式细胞仪观察常氧和缺氧条件下上皮细胞动力周期的变化 ,采用原位杂交的方法检测iNOSmRNA的变化。结果 :缺氧条件下上皮细胞的动力周期延长 ;在缺氧 3h后iNOSmRNA水平开始升高 ,12h达高峰 ,2 4h后下降 ,4 8h几乎消失 ;加入地塞米松后 ,降低这种升高的水平。但 4 μg L以上的浓度 ,并不进一步降低被缺氧升高的iNOSmRNA水平。结论 :缺氧诱发鼻粘膜上皮细胞合成高水平的iNOSmR NA ,应用一定浓度的地塞米松能降低这种作用
Objective: To investigate the changes of nasal mucosal epithelial cells in response to local hypoxia, the changes in the synthesis and secretion of inducible nitric oxide synthase (iNOS), and dexamethasone on the impact and significance. Methods: Human nasal mucosal epithelial cells were cultured in serum-free primary cells under normoxia and hypoxia conditions. The cells were incubated with different concentrations of dexamethasone. Flow cytometry was used to observe the effects of normoxia and hypoxia on epithelial cells Dynamic cycle changes, using in situ hybridization method to detect changes in iNOSmRNA. Results: After hypoxia for 3h, the iNOS mRNA level began to increase, peaked at 12h, decreased after 24 h and almost disappeared at 48 h. After dexamethasone administration, the increase of iNOS mRNA level decreased Level. However, concentrations above 4 μg L did not further reduce the level of iNOS mRNA that was elevated by hypoxia. CONCLUSION: Hypoxia induces nasal mucosal epithelial cells to synthesize high levels of iNOSmR NA, and dexamethasone at a certain concentration can reduce this effect