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目的:探讨高渗氯化钠羟乙基淀粉40注射液(HSH)对失血性休克大鼠脾脏NF-κB蛋白表达及凋亡的影响。方法:采 用SD大鼠失血性休克模型,动物随机分为高渗氯化钠羟乙基淀粉40注射液组(HSH组n=18)、林格氏液组(RL组n=18)、 假手术组(S组n=6),HSH组和RL组各分为3个亚组,分别于复苏后1．2,4h处死动物,用HE染色,透射电镜及TUNEL 法观察动物脾脏中淋巴细胞凋亡情况,同时使用免疫组化法测定脾脏中NF-κB蛋白的表达。结果:RL组1h点、HSH组各 时间点凋亡指数(Al)与S组比较无明显差异(p>0．05)。RL组2h、4h点Al高于其余组(p<0．05)．HSH各时间点Al无明 显差异(p．>0.05)。RL组除1h点外均明显高于HSH组(p<0.05)。S组、HSH组的NF-κB阳性细胞百分比均高于RL组(p <0.05)。结论:HSH复苏上调NF-KB蛋白表达,减少失血性休克导致的脾脏淋巴细胞的过度凋亡,对失血性休克后机体免 疫功能起保护作用。
Objective: To investigate the effect of hypertonic sodium chloride hydroxyethyl starch 40 injection on spleen NF-κB protein expression and apoptosis in hemorrhagic shock rats. Methods: The model of hemorrhagic shock was established in SD rats. The animals were randomly divided into Hypertonic Sodium Chloride Hydroxyethyl Starch 40 Injection group (n = 18 in HSH group), Ringer’s solution group (n = 18 in RL Group) The rats in operation group (n = 6 in S group), HSH group and RL group were divided into three subgroups. Animals were sacrificed at 1.2 and 4 hours after resuscitation. The spleen lymphocytes were observed by HE staining, transmission electron microscopy and TUNEL. At the same time, immunohistochemistry was used to detect the expression of NF-κB in the spleen. Results: The apoptosis index (AI) in RL group was not significantly different from that in S group at 1 hour after HSH (P> 0.05). Al at 2 h and 4 h in RL group was higher than the other groups (p <0.05). There was no significant difference in Al between HSH at different time points (p> 0.05). The RL group was significantly higher than the HSH group except 1h (p <0.05). The percentage of NF-κB positive cells in S group and HSH group was higher than that in RL group (p <0.05). CONCLUSIONS: HSH resuscitation up-regulates the expression of NF-κB, reduces excessive apoptosis of spleen lymphocytes induced by hemorrhagic shock, and plays a protective role in immune function after hemorrhagic shock.