运用差异展示（ＤＤ－ＲＴ－ＰＣＲ）方法对野败型（ｗｉｌｄ ａｂｏｒｔｉｏｎ）细胞质雄性不育系珍汕９７Ａ及其相应的保持系珍汕９７Ｂ基因产物进行比较分析，发现这两个核基因组完全相同的品系间约有１０％的基因表达有差异．选取一个差异表达明显的ｃＤＮＡ成员Ｂ１３２进行Ｎｏｒｔｈ－ｅｒｎ杂交发现，该基因在保持系中正常表达，在不育系中表达受到明显抑制，而在杂种一代中又正常表达，在恢复系中表达水平也很低，这是一种典型的核质互作现象．Ｓｏｕｔｈｅｒｎ杂交中，Ｂ１３２在ＨｉｎｄⅢ和ＢａｍＨⅠ酶切长度片段上，各品系间未呈现多态性． Comparative analysis of wild abortion cytoplasmic male sterile lines Zhenshan 97A and its maintainer line Zhenshan 97B by differential display (DD-RT-PCR) showed that the two nuclear genomes were identical There are about 10% of the gene expression difference between the strains. A differential expression of a significant cDNA member B132 North-ern hybridization found that the gene was normally expressed in the maintainer line, expressed in the male sterile lines were significantly inhibited, and in the hybrids and normal expression in the restorer line expression level Is also very low, this is a typical phenomenon of nuclear matter interaction. Southern hybridization, B132 in Hind Ⅲ and BamH Ⅰ digestion length fragments, the strains showed no polymorphism.