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Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin Layer Chromatography (TLC). Localization of transforming growth factor (TGF)-βI was studied by immunohistochemistry and the expression of the mRNA of TGF-βI was studied by Reverse transcriptase Polymerase Chain Reaction (RT-PCR) in uterine tissue from day 2 to day 6 of gestation. Results The TLC fraction treated in a dose of 5 mg/kg body weight per day showed decreased the number of implantation sites. The expression of TGF-βI from day 2 to day 6 of gestation was altered by the TLC fraction and suppression of the mRNA transcript of the TGF-βI on day 6 of gestation during implantation. Conclusion The root extract of Polygonum hydropiper in a dose of 5 mg/kg body weight per day decreased the expression of TGF-βI in the implantation sites of rat uterus.
Objective To examine the effect of the root extract on the growth factor during early pregnancy from day 2 to day 6 of gestation. Methods A purified form of root extract of Polygonum hydropiper was obtained by Thin Layer Chromatography (TLC). Localization of transforming growth factor (TGF) -βI was studied by immunohistochemistry and the expression of the mRNA of TGF-βI was studied by Reverse transcriptase Polymerase Chain Reaction (RT-PCR) in uterine tissue from day 2 to day 6 of gestation. Results The TLC fraction treated in a dose of 5 mg / kg body weight per day showed decreased the number of implantation sites. The expression of TGF-βI from day 2 to day 6 of gestation was altered by the TLC fraction and suppression of the mRNA transcript of the TGF-βI on day 6 of gestation during implantation. Conclusion The root extract of Polygonum hydropiper in a dose of 5 mg / kg body weight per day decreased the expression of TGF- βI in the implantation sites of rat uterus.