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目的探讨胰岛素样生长因子I型受体(insulin-like growth factor I receptor,IGF-I R) 在人结肠癌细胞株HT-29上的表达,以及两种胰岛素样生长因子I型受体单克隆抗体(IGF-I R McAb)对HT-29细胞增殖的影响。方法免疫组织化学法检测HT-29的IGF-I R表达,MTT法检测两种IGF-I R McAb对HT-29的抑制增殖作用及诱导凋亡情况。结果人结肠癌细胞株HT-29 细胞膜高表达IGF-I R。IGF-I R McAb能抑制HT-29细胞增殖,McAb对HT-29细胞的抑制作用随抗体浓度增大而增大。IGF-I R McAb诱导HT-29凋亡,与对照组相比差异有统计学意义 (P<0．01)。结论IGF-I R McAb通过阻断IGF-I R抑制结肠癌细胞增殖、诱导细胞凋亡。
Objective To investigate the expression of insulin-like growth factor I receptor (IGF-IR) on human colon cancer cell line HT-29 and the effects of two insulin-like growth factor receptor type I receptor monoclonal Antibody (IGF-I R McAb) on HT-29 Cell Proliferation. Methods Immunohistochemistry was used to detect the expression of IGF-IR in HT-29 cells. MTT assay was used to detect the proliferation and apoptosis of HT-29 induced by two IGF-I R McAbs. Results Human colon cancer cell line HT-29 cell membrane highly expressed IGF-IR. IGF-I R McAb can inhibit the proliferation of HT-29 cells, and the inhibitory effect of McAb on HT-29 cells increased with the increase of antibody concentration. The apoptosis of HT-29 induced by IGF-I R McAb was significantly different from the control group (P <0.01). Conclusion IGF-I R McAb can inhibit the proliferation of colon cancer cells by blocking IGF-I R and induce apoptosis.