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采用流式细胞仪测定Ｔ细胞亚群和脾脏Ｂ淋巴细胞电泳对应用活化剂ＨＹＰ协同“４２５”制剂的日本血吸虫感染的ＢＡＬＢ／ｃ小鼠进行细胞和体液免疫机能测定，并与单一用“４２５”制剂和感染对照动物进行比较。实验结果表明，ＨＹＰ协同“４２５”制剂应用后２ｗｋ，相当于感染后６ｗｋ，ＣＤ４＋／ＣＤ８＋淋巴细胞亚群的比值为６．８２，显著高于后两组的１．８７和１．７９，脾脏中Ｂ淋巴细胞总频率达７５％，高于单一用“４２５”制剂和感染对照组的６２．５％和６４．５％，特异性抗体水平（ＯＤ均值）为０．５５±０．１１，分别高于后两组的０．３５±０．０３和０．３７±０．０７。应用后３～４ｗｋ，相当于感染后７～８ｗｋ，ＣＤ４＋／ＣＤ８＋比值为１．９８～３．４７，后两组则分别为１．３２～１．５９和１．４８～１．７０，Ｂ淋巴细胞数３组均相近，但前组抗体水平（ＯＤ均值）的升高率（８２．２２％～９１．１８％）仍高于后两组。由此提示，活化剂ＨＹＰ协同“４２５”制剂诱导出的抗日本血吸虫虫卵肉芽肿的免疫效应，显著减弱血吸虫虫卵肉芽肿病变是与增强其细胞和体液免疫功能密切相关，具有明显的理论意义，且为进一步探索抗肉芽肿病变提供了重要依据
Flow Cytometry T cell subsets and splenic B lymphocyte electrophoresis BALB / c mice infected with Schistosoma japonicum using activator HYP in combination with “425” were tested for cellular and humoral immune function and compared with single “425 ”The formulation and infection control animals were compared. The results showed that the ratio of CD4 + / CD8 + lymphocyte subsets of HYP in combination with “425” 2wk, equivalent to 6wk after infection, was 6.82, significantly higher than that of the latter two groups (1.87 and 1.79) The overall frequency of B lymphocytes was 75%, higher than 62.5% and 64.5% of the single “425” formulation and the infected control group, and the specific antibody level (OD mean) was 0.55 ± 0.11, Respectively higher than the latter two groups of 0.35 ± 0.03 and 0.37 ± 0.07. After 3 to 4 weeks of application, it was equivalent to 7 to 8 weeks after infection, with a ratio of CD4 + / CD8 + 1.98 to 3.47, while the latter two groups were 1.32 to 1.59 and 1.48 to 1.70, respectively The number of lymphocytes in three groups was similar, but the increase rate of antibody level (OD) in the former group was still higher than the latter two groups (82.22% ~ 91.18%). It is suggested that the activation of HYP in combination with the “425” preparation induced immunity against Schistosoma japonicum egg granuloma, significantly attenuated Schistosoma japonicum egg granulomatous lesions are closely related to enhance their cellular and humoral immune function, with obvious theory Significance, and provide an important basis for further exploration of anti-granuloma lesions