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以番茄灰叶斑病为研究对象,采用形态学鉴定、致病性检测和分子生物学等方法,研究了赤峰地区番茄灰叶斑病的病原菌种类,以期为该病害的防治提供参考依据。结果表明:病原菌(ZTWYF16081515)分生孢子淡褐色,长方形至圆柱形,大小为(21.4~71.9)μm×(10.5~27.3)μm。分离菌株喷雾接种,7d后番茄叶片上形成典型的番茄灰叶斑病病斑。核糖体RNA基因内转录间隔区(ITS)和gpd区的PCR产物经测序后进行BLAST分析,均表明该菌与茄匍柄霉S.solani的ITS和gpd序列100%相同,确定该病原为茄匍柄霉S.solani。
Taking tomato gray leaf spot as the research object, the pathogen of tomato gray leaf spot in Chifeng was studied by morphological identification, pathogenicity detection and molecular biology, in order to provide a reference for the prevention and control of the disease. The results showed that the conidia of pathogen (ZTWYF16081515) were light brown, rectangular to cylindrical with the size of (21.4 ~ 71.9) μm × (10.5 ~ 27.3) μm. The isolated strains were inoculated by spraying. After 7 days, tomato leaf spots formed typical tomato gray leaf spot disease. BLAST analysis of the PCR products of ribosomal RNA internal transcribed spacer (ITS) and gpd region showed that the strain was identical to the ITS and gpd sequences of S. solani, Stubble S.solani.