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目的了解淡色库蚊对6种常用化学杀虫剂的交互抗性,为科学使用化学杀虫剂提供依据。方法采用WHO生物测定方法检测淡色库蚊敏感品系、敌敌畏抗性品系和氯氰菊酯抗性品系Ⅳ龄幼虫对三氯杀虫酯、敌敌畏、马拉硫磷、残杀威、氯氰菊酯和溴氰菊酯等6种常用化学杀虫剂的敏感性,每次测定设置7个浓度,每个浓度设2组,每组25个幼虫,24h后统计幼虫死亡数据,计算致死中浓度(median lethal concentration,LC_(50))、回归方程和抗性指数。结果淡色库蚊敌敌畏抗性品系对三氯杀虫酯、敌敌畏、马拉硫磷、残杀威、氯氰菊酯和溴氰菊醑的LC_(50)分别为1.9623mg/L、1.1607mg/L、0.7359mmg/L、0.9002mg/L、0.0220mg/L和0.000 1 mg/L,回归方程分别为Y=3.5287+5.0254X、Y=4.6962+4.693 7X、Y=5.5051+3.853 6X、Y=5.2350+5.1476X、Y=10.4995+3.3184X和Y=13.2977+2.1683X,抗性指数分别为9.25、12.17、9.14、7.93、183.47和0.71;淡色库蚊氯氰菊酯抗性品系对三氯杀虫酯、敌敌畏、马拉硫磷、残杀威、氯氰菊酯和溴氰菊酯的LC_(50)分别为5.572 8mg/L、0.2464mg/L、0.0892mg/L、0.202 7mg/L、0.064 1 mg/L和0.008 5mg/L,回归方程分别为Y=2.7728+2.9852X、Y=7.2054+3.6260X、Y=9.475 1+4.2635X、Y=6.8106+2.6125X、Y=8.7404+3.1352X和Y=14.6951+4.6853X,抗性指数分别为26.27、2.58、1.10、1.79、534.31和40.60。结论长期使用一种化学杀虫剂会导致蚊虫产生抗药性,并对其他化学杀虫剂产生不同的交互抗性,应采取合理选择杀虫剂品种和确定使用剂量等有效措施以避免蚊虫抗药性的产生。
Objective To understand the interactive resistance of Culex pipiens pallens to six commonly used chemical insecticides to provide basis for the scientific use of chemical insecticides. Methods The WHO bioassay method was used to detect the toxicity of chlorothalonil, dichlorvos, malathion, propoxur, cypermethrin and deltamethrin to susceptible strains of Culex pipiens pallens, dichlorvos and cypermethrin resistant strains The sensitivity of the commonly used chemical insecticides was set at 7 concentrations for each assay. Two groups of 25 larvae were used for each concentration. Data on larval mortality were calculated after 24 hours and the median lethal concentration (LC_ (50) )), Regression equation and resistance index. Results The LC 50 of dichlorvos resistant to Culex pipiens pallens were 1.9623mg / L, 1.1607mg / L, 0.7359mmg /L0.9002mg/L, 0.0220mg / L and 0.0001 mg / L respectively, the regression equations were Y = 3.5287 + 5.0254X, Y = 4.6962 + 4.693 7X, Y = 5.5051 + 3.853 6X, Y = 5.2350 + 5.1476X , Y = 10.4995 + 3.3184X and Y = 13.2977 + 2.1683X respectively, and the resistance index was 9.25,12.17,9.14,7.93,183.47 and 0.71 respectively. The cypermethrin resistant lines of Culex pipiens pallens were resistant to chlorothalonil, dichlorvos, The LC 50 of thiophos, propoxur, cypermethrin and deltamethrin were 5.572 8 mg / L, 0.2464 mg / L, 0.0892 mg / L, 0.202 7 mg / L, 0.064 1 mg / L and 0.008 5 mg / L, The regression equations were Y = 2.7728 + 2.9852X, Y = 7.2054 + 3.6260X, Y = 9.475 1 + 4.2635X, Y = 6.8106 + 2.6125X, Y = 8.7404 + 3.1352X and Y = 14.6951 + 4.6853X respectively. The resistance index Respectively 26.27, 2.58, 1.10, 1.79, 534.31 and 40.60. Conclusion Long-term use of a chemical insecticide will lead to the development of resistance to mosquitoes and to different cross-resistance to other chemical pesticides. Effective measures such as the selection of pesticide varieties and the determination of dosage should be taken to avoid mosquito resistance The production.