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目的:探讨渥漫青霉素对大鼠肝星状细胞(HSC)增殖的影响。方法:用链霉蛋白酶和胶原酶灌流大鼠肝脏,Nycodenz密度梯度离心分离大鼠HSC,进行体外培养;并以MTT比色法观察渥漫青霉素对HSC增殖的影响。结果:低浓度渥漫青霉素的抑制作用不明显(P>0.05),当药物浓度上升为2.5mg/L时,抑制作用增强,呈明显药物浓度依赖关系。结论:渥漫青霉素抗肝纤维化的作用是抑制了HSC的增殖,它对于防治肝纤维化可能有一定的临床意义和应用前景。
Objective: To investigate the effect of wortmannin on the proliferation of rat hepatic stellate cells (HSC). Methods: The rat liver was perfused with pronase and collagenase, HSC was isolated by Nycodenz density gradient centrifugation and cultured in vitro. The effect of wortmannin on HSC proliferation was observed by MTT assay. Results: The inhibitory effect of low concentration of vermilion was insignificant (P> 0.05). When the drug concentration was increased to 2.5mg / L, the inhibition was enhanced and the drug concentration dependence was obvious. Conclusion: The effect of wortmannin on hepatic fibrosis is to inhibit the proliferation of HSC. It may have some clinical significance and application prospects for the prevention and treatment of hepatic fibrosis.