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目的研究热力与亚甲蓝光化学法联合应用灭活辛德毕斯病毒的效果。方法采用细胞培养法和基因检测技术,对热力与亚甲蓝光化学法联合应用灭活辛德毕斯病毒的效果进行了评价。结果经56℃水浴中热力预灭活处理1.5 h,辛德毕斯病毒滴度≤0.5 TCID50/ml,灭活对数值达到6.33 TCID50/ml;未经预灭活的辛德毕斯病毒在1μmol/L亚甲蓝+光照(MB+L)处理中随光照时间的延长,病毒残余滴度逐渐下降至检测限(TCID50/ml=0.5)。经热力灭活预处理的辛德毕斯病毒与未经预处理的病毒核酸载量之间具有相关性(R2>0.98,显著性F<0.01)。结论热力灭活预处理的辛德毕斯病毒能够反映活病毒的亚甲蓝病毒灭活效果,有望成为安全而有效的病毒灭活效果评价制品。
Objective To study the effect of heat and methylene blue photochemical inactivation of Sindbis virus. Methods The effects of heat and methylene blue photochemistry combined with inactivation of Sindbis virus were evaluated by cell culture and gene detection techniques. Results The pre-deactivation treatment at 56 ℃ for 1.5 h resulted in a Sindbis virus titer of less than or equal to 0.5 TCID50 / ml and an inactivated logarithmic value of 6.33 TCID50 / ml. Sindbis virus without preactivation was treated with 1 μmol / The virus residual titer decreased gradually to the limit of detection (TCID50 / ml = 0.5) with the increase of light time in blue + light (MB + L) treatment. There was a correlation between Sindbis virus pretreated with heat inactivation and viral load without virus pretreatment (R2> 0.98, significance F <0.01). Conclusion The Sindbis virus pretreated by heat inactivation can reflect the live virus inactivation of methylene blue virus and is expected to be a safe and effective virus inactivation evaluation product.