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目的探讨大鼠在心理应激状态下咬肌中儿茶酚-O-甲基转移酶(catechol-O-methyltransferase,COMT)表达的变化。方法将48只成年Wistar雄性大鼠随机分为实验组36只与对照组12只,实验组根据刺激时间分为实验3周组、实验6周组和恢复组各12只。实验组构建心理应激模型,实验3周组、6周组及恢复组分别于刺激3周、6周及刺激去除后6周时处死大鼠;对照组不进行刺激,与恢复组同时间点处死。解剖各组大鼠咬肌,采用荧光定量PCR法检测咬肌COMT水平。结果对照组COMT/β-actin比值为1.27±0.61,实验组为0.72±0.62,其中实验3周、6周及恢复组分别为0.10±0.04、0.05±0.01、0.77±0.34,实验组与对照组大鼠咬肌COMT水平比较差异有统计学意义(P=0.039),其中实验6周组低于对照组(P<0.05),其余各组间两两比较差异均无统计学意义(P>0.05);大鼠咬肌COMT表达水平由低到高依次为实验6周组、实验3周组、恢复组和对照组。结论应激状态下大鼠咬肌COMT水平降低,儿茶酚胺降解减少可引起肌肉血管收缩,局部血液供应障碍,引起能量代谢障碍和肌肉损伤。
Objective To investigate the changes of catechol-O-methyltransferase (COMT) expression in masseter muscle under psychological stress in rats. Methods Forty-eight adult male Wistar rats were randomly divided into experimental group (36 rats) and control group (12 rats). The experimental group was divided into experimental group (3 weeks), experimental group (6 weeks) and recovery group (12 rats) Rats in experimental group were sacrificed at 3 weeks, 6 weeks and 6 weeks after stimulation, respectively. Rats in control group were sacrificed at the same time points as those in recovery group Death. The masseter muscle of each group was dissected and the COMT level of masseter muscle was detected by fluorescence quantitative PCR. Results The ratio of COMT / β-actin in the control group was 1.27 ± 0.61 and in the experimental group was 0.72 ± 0.62, which was 0.10 ± 0.04, 0.05 ± 0.01 and 0.77 ± 0.34 in the experimental group, the 6-week experimental group and the recovery group respectively There was significant difference in the COMT level between the masseter muscles of rats (P = 0.039), in which the 6 weeks group was lower than that of the control group (P <0.05), and there was no significant difference between the other groups (P> 0.05) ). The COMT expression levels in the masseter muscle from low to high were 6 weeks experimental group, 3 weeks experimental group, recovery group and control group. CONCLUSIONS: The COMT level in the masseter muscle of rats decreased under stress, and the decrease of catecholamine degradation caused muscle vasoconstriction, local blood supply disturbance, energy metabolism disorder and muscle damage.