论文部分内容阅读
目的研究尿道周围及坐骨神经近端移植人脐带间充质干细胞(hUCMSCs)是否可以提高压力性尿失禁大鼠的尿道闭合压,改善其储尿能力。方法模拟绝经和产伤构建压力性尿失禁大鼠模型,并用漏尿点压力(LPP)、最大膀胱容积(MCC)及“模拟喷嚏”实验进行检测。将hUCMSCs分别注射移植到压力性尿失禁大鼠的尿道周围及坐骨神经近端,1个月后再次检测漏尿点压力及最大膀胱容积。结果移植后1个月,尿道周围治疗组与尿道周围模型组MCC差值差异无统计学意义(P>0.05);而尿道周围治疗组LPP增高,尿道周围模型组LPP降低,两组差值差异有统计学意义(P<0.01)。移植后1个月,坐骨神经治疗组MCC和LPP与坐骨神经模型组相比,其差值差异均无统计学意义(P值均>0.05)。结论人脐带间充质干细胞注射移植到尿道周围可以提高压力性尿失禁大鼠的尿道闭合压,但不能改善其储尿能力;而移植到坐骨神经近端短期内不能改善压力性尿失禁大鼠的储尿能力。
Objective To investigate whether the transurethral transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) around the urethra and the sciatic nerve can improve the urethral closure pressure and improve the urine storage capacity of rats with stress urinary incontinence. Methods The model of stress urinary incontinence was established in postmenopausal and birth traumatic rats. The rats were examined by LPP, MCC and simulated sneeze. The hUCMSCs were respectively transplanted into the urethra and proximal sciatic nerve in stress urinary incontinence rats. After 1 month, the urinary bladder pressure and the maximum bladder volume were measured again. Results There was no significant difference in the MCC between the urethral periurethreatement group and the periurethral model group at 1 month after transplantation (P> 0.05). However, LPP increased in the periurethral group and LPP in the periurethral model group There was statistical significance (P <0.01). There was no significant difference in the MCC and LPP scores between the sciatic nerve group and the sciatic nerve model group (P> 0.05) one month after transplantation. Conclusion Transplantation of umbilical cord mesenchymal stem cells around the urethra can improve the urethral closure pressure in stress urinary incontinence rats, but it can not improve its ability to store urine. However, transplanting the proximal umbilical cord mesenchymal stem cells into the urethra can not improve the pressure-induced urinary incontinence in a short time Urinary storage capacity.