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【目的】以典型南亚热带常绿阔叶林小坑林场土壤为研究对象,模拟2008年冰雪灾害对森林造成的损伤设置实验,分析不同林冠开度和凋落物输入量对土壤固碳微生物群落结构的影响。【方法】试验设置对照(CN)、损伤处理+移除处理枝叶(TR)、损伤处理+保留处理枝叶(TD)、未处理+添加处理枝叶(UD)4个处理,受损处理一年后,采用MiSeq高通量测序技术对土壤固碳微生物的功能基因cbbL进行测序分析。【结果】通过生物信息学及统计学分析表明,森林林冠损伤后林冠开度和凋落物输入量增加,导致土壤固碳微生物种群数量降低,多样性增加,群落结构也受到影响,亚硝化螺菌属(Nitrosospira)明显增加,成为优势种群,而原来的优势菌群慢生根瘤菌属(Bradyrhizobium)明显减少。主成分分析(PCA)表明,与对照相比,其他3个样地的土壤固碳微生物结构均发生明显改变。【结论】模拟林冠损伤处理一年后,凋落物的大量骤然输入和林冠开度增大提高了土壤固碳微生物群落多样性,但降低了其种群数量,影响了土壤固碳微生物群落结构,这为进一步的研究提供了依据。
【Objective】 The objective of this study was to investigate the damage caused by ice and snow disasters to the forest in Xiaokeng Forest Farm in a typical subtropical evergreen broad-leaved forest in China. Experiments were conducted to study the effects of different canopy opening and litter input on the soil carbon sequestration, Impact. 【Method】 Four treatments including control (CN), injury treatment + removal treatment of foliage (TR), injury treatment + retention treatment of foliage (TD), untreated + addition treatment of foliage (UD) , MiSeq high-throughput sequencing technology was used to analyze the functional gene cbbL of soil carbon sequestration microorganisms. 【Result】 Bioinformatics and statistical analysis showed that the opening of canopy and input of litter increased after forest canopy injury, which led to the decrease of population of carbon sequestration in soil, increased diversity and community structure. Nitrositis (Nitrosospira) increased significantly, becoming the dominant population, and the original dominant flora Bradyrhizobium decreased significantly. Principal component analysis (PCA) showed that compared with the control, the structure of soil carbon sequestration in the other three plots changed significantly. 【Conclusion】 A year after simulated canopy damage treatment, a large number of sudden input of litter and increased canopy opening increased the diversity of carbon sequestration in the soil, but decreased the population and influenced the structure of carbon sequestration in soil Provided the basis for further research.