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目的 探讨吸入地塞米松对气道重建和对离体平滑肌增殖作用的影响。方法 雄性豚鼠 40只 ,随机分为 4组 :哮喘组、治疗组、地塞米松组、对照组 ,每组 10只。每组动物连续 3个月雾化吸入不同药物后留取病理组织 ,采用计算机图像分析气道壁厚度。通过对犬气管离体平滑肌细胞培养 ,测定组胺 (His)组、血栓素A2 组和地塞米松组 [3H] 胸腺嘧啶核苷 ([3H] TdR)的闪烁计数值。结果 哮喘组豚鼠气管平滑肌厚度为 (90± 14) μm ,与对照组 (5 4± 7) μm比较 ,差异有显著性 (P <0 0 1) ;治疗组气管平滑肌厚度为 (6 1± 11) μm ,与哮喘组比较 ,差异有显著性 (P <0 0 1)。His组 [3H] TdR值为 (2 95 0± 2 0 5 )次 /分 ,与对照组 [(6 13± 5 2 )次 /分 ]比较 ,差异有显著性 (P <0 0 1) ;地塞米松组[3H] TdR值为 (10 6 7± 96 )次 /分 ,与His组比较差异有显著性 (P <0 0 1)。结论 地塞米松能抑制气道平滑肌细胞增殖 ,早期吸入地塞米松能够有效防治气道重建 ,对气道无损害作用
Objective To investigate the effects of dexamethasone inhalation on airway remodeling and proliferation of isolated smooth muscle in vitro. Methods Forty male guinea pigs were randomly divided into 4 groups: asthma group, treatment group, dexamethasone group and control group, with 10 in each group. Each group of animals for 3 consecutive months of inhalation of different drugs inhalation of pathological tissue, using computerized image analysis of airway wall thickness. Scintigraphy of [3H] thymidine ([3H] TdR) in histamine (His), thromboxane A2, and dexamethasone groups was determined by culturing canine tracheal in vitro smooth muscle cells. Results The thickness of tracheal smooth muscle in asthma group was (90 ± 14) μm, which was significantly different from that of control group (54 ± 7) μm (P <0.01). The thickness of tracheal smooth muscle in treatment group was (6 1 ± 11) ) μm, there was a significant difference between asthma group and control group (P <0.01). The value of [3H] TdR in His group was (2 95 0 ± 2 0 5) / min, which was significantly different from that of the control group [(6 13 ± 5 2) / min) (P 0 01). The [3H] TdR value of dexamethasone group was (10 6 7 ± 96) / min, which was significantly different from that of His group (P 0 01). Conclusion Dexamethasone can inhibit the proliferation of airway smooth muscle cells. Early inhalation of dexamethasone can effectively prevent airway remodeling and has no damage to airway