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利用颜色的变化研究反应的进行情况是应用广泛、简便而直观的方法,是有机试剂研究的重要内容。除分析化学外,对络合物化学、染料化学、结构化学以至生物化学都有重要意义,例如对吸收光谱的探讨就是从叶绿素提取物的研究开始的(D.Brewter,1834)。为了深入探究试剂的结构与络合物的显色性能以及它们的相互关系,有必要对吸收光谱进行解析。本文试对紫外-可见光谱概念、吸收光谱的解析方法及吸光系数的计算作一简介,并
The study of the reaction progress with the change of color is a widely used, simple and intuitive method, which is an important part of organic reagent research. In addition to analytical chemistry, complex chemistry, dye chemistry, structural chemistry and even biochemistry are important, for example, the study of absorption spectra from the study of chlorophyll extract (D. Brewster, 1834). In order to further explore the reagent structure and the color development of complexes and their interrelationship, it is necessary to analyze the absorption spectrum. This article attempts to make a brief introduction to the concept of ultraviolet-visible spectrum, the analytical method of absorption spectrum and the calculation of absorption coefficient