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复合氨基酸注射液是含有11种氨基酸及甘露醇的复方制剂。文献曾报导用二阶导数光谱测定蛋白质及天然物中的色氨酸;徐绪温等使用双波长分光光度计,用导数光谱法测定了复合氨基酸注射液中色氨酸,本文提出用二阶导数光谱直接测定色氨酸和苯丙氨酸含量的方法。实验方法 (一)仪器与试剂所用仪器为岛津UV-240型自记式紫外分光光度计OP1-1附件。氨基酸标准物均为广州侨光制药厂提供的日本进口试剂,按药典检定合格;甘露醇,分析纯。复合氨基酸注射液由广州侨光制药厂提供,为内含11种氨基酸及甘露醇的无菌水溶液,
Compound amino acid injection is a compound containing 11 kinds of amino acids and mannitol. The literature has reported the determination of tryptophan in proteins and natural products by second-order derivative spectroscopy. Xu Xuwen et al. Used dual-wavelength spectrophotometer to determine tryptophan in the composite amino acid injection by derivative spectroscopy. In this paper, Direct determination of tryptophan and phenylalanine content of the method. Experimental methods (a) instruments and reagents used equipment Shimadzu UV-240 self -recorded ultraviolet spectrophotometer OP1-1 annex. Amino acid standards are Guangzhou Qiaoguang Pharmaceutical Japan imported reagents, according to the Pharmacopoeia qualified; mannitol, analytical grade. Composite amino acid injection from Guangzhou Qiaopu pharmaceutical factory to provide 11 kinds of amino acids and mannitol containing sterile aqueous solution,