目的：研究ＡｒｇＧｌｙＡｓｐＳｅｒ（ＲＧＤＳ）肽对血小板聚集和血小板膜ＧＰⅡｂ／Ⅲａ反磷酸化的影响。方法：免疫沉淀提取血小板膜ＧＰⅡｂ／Ⅲａ，反磷酸化测定。结果：５０μｍｏｌ·Ｌ－１ＡＤＰ引起血小板聚集时，其ＧＰⅡｂ／Ⅲａ反磷酸化明显降低。而应用１００和２００μｍｏｌ·Ｌ－１ＲＧＤＳ肽与ＡＤＰ共同孵育，呈浓度依赖抑制血小板聚集的同时，可逆转ＡＤＰ引起的蛋白反磷酸化改变（Ｐ＜０．０１），其抑制血小板聚集和ＧＰⅡｂ／Ⅲａ反磷酸化呈明显的负相关（ｒ＝－０．７６，Ｐ＜０．０１）。结论：ＲＧＤＳ肽抑制血小板聚集是通过抑制血小板膜ＧＰⅡｂ／Ⅲａ磷酸化所致 Objective: To study the effect of ArgGlyAspSer (RGDS) peptide on platelet aggregation and GPⅡb / Ⅲa phosphorylation. Methods: Platelet membrane GPⅡb / Ⅲa was extracted by immunoprecipitation, and its anti-phosphorylation was measured. Results: When platelet aggregation was induced by 50μmol·L-1ADP, the anti-phosphorylation of GPⅡb / Ⅲa was significantly decreased. However, incubation of 100 and 200 μmol·L-1 RGDDS peptides with ADP inhibited the aggregation of platelets in a concentration-dependent manner and reversed the phosphorylation of protein induced by ADP (P <0.01), which inhibited platelet aggregation and GPⅡb / Ⅲa Anti-phosphorylation showed a significant negative correlation (r = -0.76, P <0.01). Conclusion: RGDS peptide inhibits platelet aggregation by inhibiting platelet membrane GP Ⅱ b / Ⅲ a phosphorylation