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目的探讨循环miRNA-4281在肝癌患者血清中的表达情况及对诊断的临床意义。方法运用QRT-PCR技术分别检测45例肝癌患者(肝癌组)及正常人(对照组)血清中miRNA-4281的表达量。结果与对照组相比,肝癌患者血清标本中miRNA-4281的表达量明显高于正常人,且差异有统计学意义(P<0.05)。ROC曲线下面积是0.805,microRNA-4281作为肝癌诊断的灵敏度为84.40%,特异度是73.30%。miRNA-4281的表达与AFP无明显相关性。Kaplan-Meier分析提示miRNA-4281表达水平高的患者具有较短的生存期限(P<0.05)。结论检测miRNA-4281有利于肝癌的诊断,可作为HCC潜在的临床诊断标志物,同时其对于评估患者的预后具有重要作用。
Objective To investigate the expression of circulating miRNA-4281 in serum of hepatocellular carcinoma patients and its clinical significance. Methods The serum levels of miRNA-4281 in 45 patients with hepatocellular carcinoma (HCC) and normal controls (control group) were detected by QRT-PCR. Results Compared with the control group, the expression of miRNA-4281 in serum of hepatocellular carcinoma patients was significantly higher than that in normal controls (P <0.05). The area under the ROC curve was 0.805, the sensitivity of microRNA-4281 as a diagnosis of liver cancer was 84.40%, and the specificity was 73.30%. There was no significant correlation between the expression of miRNA-4281 and AFP. Kaplan-Meier analysis suggested that patients with high miRNA-4281 expression had a shorter survival time (P <0.05). Conclusion Detection of miRNA-4281 is helpful for the diagnosis of hepatocellular carcinoma and can be used as a potential clinical diagnostic marker for HCC. It also plays an important role in assessing the prognosis of patients.