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目的与方法为了全面掌握青南高原细粒棘球绦虫的种内变异,我们首次应用mtDNA的coxI基因,nadI基因和atp6基因检测了55个细粒棘球绦虫分离株(37个人体分离株,18个动物分离株)的基因多态性。结果研究结果显示,所检测的55个细粒棘球绦虫分离株与GenBank中检索到的普通羊株(G1型)基因同源性达到90%以上,均为普通羊株(G1型)。将mtDNA相加(共1 327bp)后得到了13个基因型,均被GenBank收录。结论1)55个细粒棘球绦虫分离株均为普通羊株(G1型);2)结果所得的13个基因型中,既有世界上广泛分布的,也有本研究首次报道的,充分说明青南地区细粒棘球绦虫具有广泛性和普遍性的流行病学特征,青南高原特殊地理环境和气候条件导致细粒棘球绦虫的特殊的核苷酸变异。
Objectives and Methods In order to fully understand the intraspecific variation of Echinococcus granulosus on the Qingnan Plateau, we first detected 55 Echinococcus granulosus isolates (37 human isolates, 18 Animal isolates). Results The results showed that the homology between the 55 isolates of Echinococcus granulosus and the normal sheep (Genotype G1) retrieved from GenBank was more than 90%, all of which were normal sheep (G1 type). Thirteen genotypes were obtained after adding mtDNA (1 327 bp in total), all of which were included in GenBank. CONCLUSIONS: 1) All 55 Echinococcus granulosus isolates were normal goats (G1 type); 2) Among the 13 genotypes obtained, they were widely distributed in the world and were also reported for the first time in this study The Echinococcus granulosus in southern Qinghai Province has extensive and universal epidemiological characteristics. The special geographical variation and climatic conditions lead to the special nucleotide variation of Echinococcus granulosus in Qingnan Plateau.