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采用铜镉柱还原硝酸盐,与CdTe量子点增敏过氧亚硝酸-碳酸钠体系的化学发光信号相结合,开发了快速在线同时分析亚硝酸盐和硝酸盐的新方法.对流动注射、化学发光等实验参数条件进行优化,在Na2CO3的浓度为0.2 M、H2O2的浓度为0.03 M、Na2EDTA的浓度为1×10-3 M、CdTe量子点粒径为2.84 nm的条件下,过氧亚硝酸-碳酸钠体系可以获得最优的化学发光信号.该方法检测亚硝酸盐的线性范围为0.3~75μM,检测限可达0.12μM,其相对标准偏差为1.9%;硝酸盐的线性范围为1.0~100μM,检测限可达0.26μM,其相对标准偏差为1.5%.此方法无需衍生和分离,可以实现同时、准确、快速和高选择性地检测人体尿液中亚硝酸盐和硝酸盐的含量,回收率分别为94%~105%和96.6%~110.4%.
A new method of simultaneous on-line and simultaneous analysis of nitrite and nitrate was developed by reducing the nitrate with copper-cadmium column and chemiluminescence signal of CdTe QD-sensitive peroxynitrite-sodium carbonate system. Luminescence and other experimental parameters were optimized under the conditions of Na2CO3 concentration of 0.2 M, H2O2 concentration of 0.03 M, Na2EDTA concentration of 1 × 10-3 M, CdTe quantum dots particle size of 2.84 nm, the peroxynitrite - sodium carbonate system can obtain the best chemiluminescence signal.The detection method of nitrite linear range of 0.3 ~ 75μM, the detection limit of up to 0.12μM, the relative standard deviation of 1.9%; nitrate linear range of 1.0 ~ 100μM with a detection limit of 0.26μM and a relative standard deviation of 1.5% .The proposed method can simultaneously, accurately, rapidly and selectively detect nitrite and nitrate in human urine without derivatization and separation, The recoveries were 94% -105% and 96.6% -110.4%, respectively.