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目的探索处于弓形虫急性感染期的雌、雄小鼠的学习记忆能力的变化及其可能的机制。方法以弓形虫RH株速殖子数量300/只腹腔注射小鼠建立弓形虫急性感染期模型,利用穿梭箱实验对感染组小鼠和对照组小鼠感染后第0、1、2、3、4和5天的学习记忆能力进行评价,并从感染当天开始,每天分离各组小鼠中脑,以荧光定量PCR法对其中脑内酪氨酸羟化酶(TH)的表达水平进行检测。结果从感染后第3天开始,感染组小鼠的学习记忆能力明显低于对照组小鼠,差异有统计学意义(第3、4和5天:P<0.05)。从感染后第1天开始,感染组小鼠中脑TH表达量开始升高,感染组平均表达量相较于对照组明显升高,差异有统计学意义(P<0.05),且雌性感染组TH平均表达量高于雄性感染组,差异有统计学意义(P<0.05),而对照组之间则无明显区别(P>0.05)。结论弓形虫急性感染可损害小鼠的学习记忆能力并使其中脑TH表达水平增高,该作用具有性别差异且雌性更易受影响。
Objective To explore the changes of learning and memory ability of female and male mice at the acute stage of Toxoplasma gondii infection and its possible mechanism. Methods Toxoplasma gondii RH strain tachyzoites number 300 / only intraperitoneal injection of mice to establish a model of acute Toxoplasma infection period, the use of shuttle box infection in mice and control mice infected 0,1,2,3, 4 and 5 days of learning and memory were evaluated. From the day of infection, the midbrain of each group was separated daily, and the expression of tyrosine hydroxylase (TH) in the brain was detected by fluorescence quantitative PCR. Results From the third day after infection, the learning and memory ability of infected mice was significantly lower than that of the control mice (days 3, 4 and 5: P <0.05). From the first day after infection, the expression of TH in the brain of infected mice began to increase, and the average expression level of the infected group was significantly higher than that of the control group (P <0.05), and the female infected group The average expression level of TH was higher than that of male infected group (P <0.05), but there was no significant difference between control group (P> 0.05). Conclusion The acute infection with Toxoplasma gondii can impair the learning and memory abilities of mice and increase the expression of TH in the midbrain, which has the gender difference and the female is more likely to be affected.