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目的 研究H .pylori对体外培养的胃上皮细胞增殖与凋亡的影响。方法 以SGC 790 1细胞作为H .pylori感染的体外细胞模型 ,用Ki 6 7抗原的免疫组化分析检测了H .pylori标准菌株NCTC 116 37活菌对胃上皮细胞增殖的影响 ,同时用流式细胞术、荧光染色技术检测了细胞凋亡率。结果 H .pylori在较低浓度 (≤ 1.6× 10 5CFU/ml)时对细胞增殖有促进作用 ,而在较高浓度 (≥ 8× 10 5CFU/ml)时抑制细胞增殖。H .pylori以浓度依赖方式诱导胃上皮细胞凋亡 ,Hoechst 332 5 8荧光染色和流式细胞术两种方法所得结果一致。结论 细胞凋亡与增殖间的不平衡亦可部分解释人体感染H .pylori后所表现的多样化结局
Objective To study the effect of H.pylori on the proliferation and apoptosis of gastric epithelial cells cultured in vitro. Methods The in vitro cell model of SGC 7901 cells infected with H. pylori was used to detect the effect of Hp. Pylori standard strain NCTC 116 37 on the proliferation of gastric epithelial cells by immunohistochemical analysis of Ki 6 7 antigen. Cytometry and fluorescence staining were used to detect the apoptosis rate. Results H. pylori promoted cell proliferation at lower concentrations (≤ 1.6 × 10 5 CFU / ml) and inhibited cell proliferation at higher concentrations (≥ 8 × 10 5 CFU / ml). H.pylori induced gastric epithelial cell apoptosis in a concentration-dependent manner. The results obtained by Hoechst 332 58 fluorescence staining and flow cytometry were the same. Conclusions The imbalance between apoptosis and proliferation may also partially explain the diversified outcome after human infection with H.pylori