作者曾报道,酶标醋酸纤维膜免疫电泳技术检测长叶车前花叶病毒为0.3ng样品量。如不用醋酸纤维膜为载体,而用广泛使用的琼脂糖凝胶为载体,也能提高检测的灵敏度。 1.长叶车前花叶病毒抗原的纯化及其免疫球蛋白的制备(请参阅朱培坤:《免疫酶技术》一书)。 2.酶标抗体的制备:采用戊二醛——过碘酸钠氧化法,将辣根过氧化物酶溶于碳酸盐缓冲液,加 The authors have reported, enzyme-labeled acetate membrane immunoelectrophoresis technology for the detection of long-leaved mosaic virus is 0.3ng sample volume. If not using cellulose acetate membrane as a carrier, and using the widely used agarose gel as a carrier, can also improve the detection sensitivity. 1. Purification of long-leaf car front mosaic virus antigen and its immunoglobulin preparation (see Zhu Peikun: “Enzyme Technology” book). 2. Preparation of enzyme-labeled antibody: using glutaraldehyde - sodium periodate oxidation method, the horseradish peroxidase was dissolved in carbonate buffer, plus