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用不同浓度的苯(1,2,3)噻二嗪-7-硫代羧酸-S-甲酯(BTH)或2,6-二氯异烟酸(INA)喷雾处理小麦幼苗,进行小麦诱导抗病性研究。结果表明,以1.0mmol(毫摩尔)INA处理后接种小麦条锈病的诱导抗性效果最好。以1.0mmolINA和0.3mmolBTH处理小麦叶片5d后接种,叶片中苯丙氨酸解氨酶(PAL)和多酚氧化酶(PPO)的活性均有明显上升,且PAL、PPO和INA、BTH的诱导抗性表达都呈正相关关系。BTH和INA水溶液对小麦条锈菌夏孢子的萌发均无明显抑制作用。据此认为,处理后病情指数的下降是由于试剂处理提高了小麦幼苗的抗病性,即麦苗产生了诱导抗性而引起的。
Wheat seedlings were sprayed with different concentrations of benzene (1, 2, 3) thiadiazine-7-thiocarboxylic acid-S-methyl ester (BTH) or 2,6- dichloroisonicotinic acid (INA) Induced resistance studies. The results showed that induction of stripe rust inoculated with 1.0 mmol (mmole) INA gave the best induction resistance. The activities of PAL and PPO in leaves were significantly increased after inoculation with 1.0 mmolINA and 0.3 mmol BTH for 5 d, and the induction of PAL, PPO and INA and BTH Resistance expression showed a positive correlation. BTH and INA aqueous solution had no significant inhibitory effect on the spore germination of wheat stripe rust. Therefore, the decline of the disease index after treatment is due to the fact that the reagent treatment raises the disease resistance of wheat seedlings, that is, the induced resistance of wheat seedlings.