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为探讨球襄损伤动脉后血管壁细胞增殖和凋亡与P53、Bcl-2、c－Myc和c－Fos等癌蛋白表达时相的关系．将贵州小型猪喂高胆固醇饲料合并球襄损伤诱导动脉粥样硬化病变，然后再行右髂动脉球襄血管成形术．水后1、3、8、14、30和90天，分别用抗增殖细胞核抗原抗体标记检测细胞增殖；用未端脱氧核苷酸转移酶介导的dUTP-生物素平移末端标记检测细胞凋亡；p53、Bcl-2、c－Myc和c－Fos蛋白表达的检测用特异性及单克隆抗体标记和用亲和素-生物素-过氧物酶结合过氧化物酶度物3-3’二氨基联苯胺对组织切片进行免疫组织化学染色．结果发现，内膜和中膜在损伤后第1、3和8天时增殖细胞核抗原阳性细胞百分率分别为224％对21．6％、27．8％对27．5％，30．6％对21．2％；生物素平移末端标记阳性细胞百分率分别为3．0％对0．9％，4．3％对1．4％、16．0％对1．9％。C－Myc和c－Fos蛋白表达增加，与增殖细胞核抗原的直线相关系数分别为0．6472和0．6362。内膜和中膜的Bcl-2蛋白表达下调，而P53蛋白表达上调，与生物素平移末端标记的直线相关系数分别为为-0．7538和0．8447。增殖细胞核抗原、生物素平移末端标记、C－Myc、c－Fos、Bcl－2和P53阳性细胞百分数在损伤与未损伤血管以及在内膜和中膜的检测结果均有所不同。结论提?
To investigate the relationship between the proliferation and apoptosis of vascular wall cells and the expression of P53, Bcl-2, c-Myc, c-Fos and other cancer proteins. The Guizhou minipigs were fed high cholesterol diets with balloon injury to induce atherosclerotic lesions, followed by right radial artery balloon angioplasty. At 1, 3, 8, 14, 30, and 90 days after water, cell proliferation was detected by anti-proliferating cell nuclear antigen antibody labeling, and cell apoptosis was detected by dUTP-biotin translational end-labeling mediated by a terminal deoxynucleotidyl transferase. Detection of p53, Bcl-2, c-Myc, and c-Fos protein expression using specific and monoclonal antibodies and binding to peroxidase 3-3 by avidin-biotin-peroxidase Diaminobenzidine immunohistochemically stained tissue sections. The results showed that the percentage of proliferating cell nuclear antigen-positive cells in the intima and media at the 1st, 3rd, and 8th day after injury was respectively 224% vs. 21.6%, 27.8% vs 27.5%, and 30.6% vs 21 The percentage of biotin-translating end-labeling positive cells was 3.0%, 0.9%, 4.3% versus 1.4%, and 16.0% versus 1.9%, respectively. The expression of C-Myc and c-Fos protein increased, and the linear correlation coefficients with proliferating cell nuclear antigen were 0.6472 and 0.6362, respectively. The expression of Bcl-2 protein was down-regulated in intima and media, while the expression of P53 protein was up-regulated. The linear correlation coefficients with biotin-translated end-labels were -0.7538 and 0.8447, respectively. Percentages of proliferating cell nuclear antigen, biotin translating end labeling, C-Myc, c-Fos, Bcl-2, and P53 positive cells were different in injured and uninjured blood vessels and in the intima and media. Conclusion